Defining conditions for biofilm inhibition and eradication assays for Gram-positive clinical reference strains.
Cristina D CruzShreya ShahPäivi TammelaPublished in: BMC microbiology (2018)
In summary, we defined conditions for optimal biofilm growth and for standardized resazurin assay for MBIC determination against six Gram-positive clinical reference strains. We also observed that MBEC determination by the resazurin-based assay is limited due to the poor detection limit of the assay. Complementary cell counting data is needed for precise determination of MBEC.
Keyphrases
- high throughput
- solid phase extraction
- pseudomonas aeruginosa
- escherichia coli
- staphylococcus aureus
- gram negative
- molecularly imprinted
- candida albicans
- single cell
- biofilm formation
- electronic health record
- multidrug resistant
- big data
- mesenchymal stem cells
- helicobacter pylori infection
- mass spectrometry
- cystic fibrosis
- quantum dots
- sensitive detection