Rapid sensing and imaging of methylglyoxal in living cells enabled by a near-infrared fluorescent probe.

Hai XuXin-Rong LiuZheng-Hong CaiJianfeng ZhengYa-Wen WangYu Peng

Published in: Organic & biomolecular chemistry (2022)

A novel near-infrared fluorescent probe (SWJT-2) has been designed and synthesized for the detection of methylglyoxal (MGO). It showed a low detection limit (0.32 μM), high selectivity and the fastest detection (15 min) over various reactive carbonyl compounds in aqueous solution. SWJT-2 had been successfully applied to bioimaging in HeLa cells to detect exogenous and endogenous MGO.

Keyphrases

fluorescent probe
living cells
loop mediated isothermal amplification
single molecule
aqueous solution
label free
real time pcr
cell cycle arrest
induced apoptosis
high resolution
sensitive detection
photodynamic therapy
pi k akt
fluorescence imaging