Activity of arginine-phenylalanine and arginine-tryptophan-based surfactants against <i>Staphylococcus aureus</i>.
Letícia Ss MorenoFrancisca Bsa NascimentoCecília R da SilvaLívia Gav SáJoão Ba NetoJacilene SilvaEmmanuel M SilvaHelcio S Dos SantosLourdes PérezAnderson R da SilvaDaniel S RodriguesAmanda D BarbosaLara Ea MoreiraBruno C CavalcantiManoel O de MoraisHélio Vitoriano Nobre JúniorPublished in: Future microbiology (2022)
<b>Aims:</b> This study aimed to evaluate the antibacterial effect of two new cationic surfactants based on phenylalanine-arginine (LPAM) and tryptophan-arginine (LTAM). <b>Materials & methods:</b> Antibacterial activity, mechanism of action and interactions with <i>Staphylococcus aureus</i> enzymes were measured through microbiological, flow cytometry and molecular docking assays, respectively. <b>Results & conclusion:</b> These compounds showed antibacterial activity in the range of 4.06-16.24 μg/ml against planktonic cells and no activity against mature biofilms, since they caused a loss of membrane integrity and increased DNA damage, as revealed by flow cytometry analysis. <i>In silico</i> assays revealed the existence of molecular bonds such as hydrogen bonds, mainly with DNA. Therefore, these compounds have promising pharmacological activity against MRSA strains.
Keyphrases
- flow cytometry
- staphylococcus aureus
- molecular docking
- nitric oxide
- dna damage
- high throughput
- methicillin resistant staphylococcus aureus
- protein kinase
- oxidative stress
- silver nanoparticles
- amino acid
- molecular dynamics simulations
- biofilm formation
- escherichia coli
- induced apoptosis
- single molecule
- candida albicans
- cell proliferation
- dna repair
- cell cycle arrest