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Clinical Performance of Lateral Flow Assay for Cryptosporidium spp. Diagnosis.

Miriam Campos-RuizClara FlamarichAnabel Fernández-NavarroSilvia RouraLaura MartinPablo PilladoPere Joan CardonaGema Fernández-Rivas
Published in: Biomedicines (2023)
Cryptosporidium spp. is an apicomplexan protozoan parasite associated with gastroenteritis in humans. In 2018, Spain showed 1511 confirmed cases, with a growing trend since 2014. Despite this fact, Cryptosporidium spp. is not usually routinely examined when a parasitological study is ordered, although accurate diagnosis is fundamental to prevent the spread of the illness. The main objectives of the present work is to demonstrate the circulation and to study the epidemiology of cryptosporidiosis in patients who were being tested for the presence of Cryptosporidium spp. parasites in the faeces in the Metropolitan North Area of Barcelona, Maresme, and Vallés Occidental using a two-step algorithm. The stool samples were analysed using the Cryptosporidium / Giardia spp. immunochromatographic test; the positive samples were visualised under a microscope using auramine staining. The proportion of Cryptosporidium spp. cases was around 2% in the studied patients, with a pronounced seasonal incidence peak in late summer-early autumn. In our cohort, weight loss was the main symptom related to confirmed cases. The mean age of confirmed patients was 19 years old, and they were younger than the unconfirmed group. Cryptosporidium spp. is one of the parasites that currently circulate in many areas in Europe. Prevalence must be taken into account for active searching.
Keyphrases
  • risk factors
  • weight loss
  • end stage renal disease
  • newly diagnosed
  • chronic kidney disease
  • plasmodium falciparum
  • high throughput
  • peritoneal dialysis
  • body mass index
  • solid state