Dispersion of SARS-CoV-2 RNA across a wastewater treatment plant and its workers.

Within urban and suburban sewersheds, SARS-CoV-2 released through faeces is transported through sewage systems into municipal wastewater treatment plants (WWTPs). Studies have shown that viral RNA is detectable in untreated wastewater but not in WWTP effluent. In this study, we investigated treatment steps between the influent and final treated effluent to identify the point at which viral RNA is below detection. Additionally, we examined air surrounding high turbulence treatment steps to test for the presence of SARS-CoV-2 RNA in WWTP-generated bioaerosols. To examine potential worker exposure to SARS-CoV-2, WWTP workers were tested for the presence of viral RNA. The data show that despite high viral RNA concentration in the influent, SARS-CoV-2 RNA concentration decreased significantly ( p  < 0.02) in the main treatment steps and was below detection in the effluent. Additionally, SARS-CoV-2 RNA was below detection in air samples ( n =  42), and the worker rate of infection was not significantly different ( p  = 0.99) from the rate of infection in the surrounding community. These results suggest that WWTP workers may have minimal exposure to SARS-CoV-2 during routine outdoor work procedures and that the WWTP successfully reduces the amount of viral RNA entering effluent receiving waters, providing a vital public health service to communities.