Standardization of Semi-Quantitative Dot Blotting Assay-Application in the Diagnosis, Follow-Up, and Relapse of Paracoccidioidomycosis.

Beatriz Aparecida Soares PereiraRicardo de Souza CavalcanteVera Lucia Pereira-Chioccola Marcia de Souza Carvalho MelhemLídia Raquel de CarvalhoRinaldo Pôncio Mendes

Published in: Microorganisms (2024)

A DB assay is easier and faster than a DID test to be performed; DB and DID tests show the same accuracy, while blood qPCR is not recommended in the diagnosis at the time of admission; cross-reactions were not observed with other systemic diseases; DB and DID tests are useful for treatment monitoring PCM patients; and a DB assay is the choice for diagnosing relapse. These findings support the introduction of semi-quantitative DB assays in clinical laboratories.

Keyphrases

high throughput
end stage renal disease
newly diagnosed
ejection fraction
prognostic factors
peritoneal dialysis
mass spectrometry
quantum dots
single cell