Supramolecular Photoactivatable Anticancer Hydrogels.
V VenkateshNarendra Kumar MishraIsolda Romero-CanelónRobbin R VernooijHuayun ShiJames P C CoverdaleAbraha HabtemariamSandeep VermaPeter J SadlerPublished in: Journal of the American Chemical Society (2017)
A photoactivatable dopamine-conjugated platinum(IV) anticancer complex (Pt-DA) has been incorporated into G-quadruplex G4K+ borate hydrogels by using borate ester linkages (Pt-G4K+B hydrogel). These were characterized by 11B NMR, attenuated total reflection Fourier transform infrared spectroscopy, circular dichroism, scanning electron microscopy and transmission electron microscopy. Microscopy investigations revealed the transformation of an extended fiber assembly into discrete flakes after incorporation of Pt-DA. Pt-DA showed photocytotoxicity against cisplatin-resistant A2780Cis human ovarian cancer cells (IC50 74 μM, blue light) with a photocytotoxic index <2, whereas Pt-G4K+B hydrogels exhibited more potent photocytotoxicity (IC50 3 μM, blue light) with a photocytotoxic index >5. Most notably, Pt-DA and Pt-G4K+B hydrogels show selective phototoxicity for cancer cells versus normal fibroblast cells (MRC5).
Keyphrases
- electron microscopy
- drug delivery
- hyaluronic acid
- wound healing
- tissue engineering
- high resolution
- drug release
- extracellular matrix
- endothelial cells
- magnetic resonance
- induced apoptosis
- optical coherence tomography
- high throughput
- photodynamic therapy
- oxidative stress
- cell proliferation
- cell death
- signaling pathway