Identification and characterization of plasmids carrying the mobile colistin resistance gene mcr-1 using optical DNA mapping.
Sriram K KMoa S WranneTsegaye SewunetElina EkedahlMaarten CoorensTeerawit TangkoskulVisanu ThamlikitkulChristian G GiskeFredrik WesterlundPublished in: JAC-antimicrobial resistance (2023)
Both ODM and the cut-and-stretch assay developed could be very useful in identifying plasmids encoding antibiotic resistance in hospitals and healthcare facilities. The cut-and-stretch assay is particularly useful in low- and middle-income countries, where existing techniques are limited.
Keyphrases
- escherichia coli
- klebsiella pneumoniae
- healthcare
- high resolution
- high throughput
- multidrug resistant
- genome wide
- copy number
- single molecule
- cell free
- pseudomonas aeruginosa
- high speed
- acinetobacter baumannii
- genome wide identification
- dna methylation
- transcription factor
- mass spectrometry
- gene expression
- single cell