Evaluation of High-Resolution Mass Spectrometry for the Quantitative Analysis of Mycotoxins in Complex Feed Matrices.
Tolke JensenMarthe De BoevreNils PreußkeSarah De SaegerTim BirrJoseph-Alexander VerreetFrank D SönnichsenPublished in: Toxins (2019)
The selective and sensitive analysis of mycotoxins in highly complex feed matrices is a great challenge. In this study, the suitability of OrbitrapTM-based high-resolution mass spectrometry (HRMS) for routine mycotoxin analysis in complex feeds was demonstrated by the successful validation of a full MS/data-dependent MS/MS acquisition method for the quantitative determination of eight Fusarium mycotoxins in forage maize and maize silage according to the Commission Decision 2002/657/EC. The required resolving power for accurate mass assignments (<5 ppm) was determined as 35,000 full width at half maximum (FWHM) and 70,000 FWHM for forage maize and maize silage, respectively. The recovery (RA), intra-day precision (RSDr), and inter-day precision (RSDR) of measurements were in the range of 94 to 108%, 2 to 16%, and 2 to 12%, whereas the decision limit (CCα) and the detection capability (CCβ) varied from 11 to 88 µg/kg and 20 to 141 µg/kg, respectively. A set of naturally contaminated forage maize and maize silage samples collected in northern Germany in 2017 was analyzed to confirm the applicability of the HRMS method to real samples. At least four Fusarium mycotoxins were quantified in each sample, highlighting the frequent co-occurrence of mycotoxins in feed.
Keyphrases
- high resolution mass spectrometry
- liquid chromatography
- ultra high performance liquid chromatography
- ms ms
- mass spectrometry
- gas chromatography
- rheumatoid arthritis
- tandem mass spectrometry
- high resolution
- multiple sclerosis
- electronic health record
- clinical practice
- drinking water
- risk assessment
- ankylosing spondylitis
- artificial intelligence
- sensitive detection
- clinical evaluation