Solvent effects on differentiation of mouse brain tissue using laser microdissection 'cut and drop' sampling with direct mass spectral analysis.

John F Cahill Vlimos Kertesz Tiffany Porta SiegelJ C Yves LeBlancRon M A Heeren Gary J Van Berkel

Published in: Rapid communications in mass spectrometry : RCM (2018)

Combined TOF-MS and SWATH-MS data differentiated white, grey, granular, and nucleus mouse tissue regions with greater accuracy than when solely using TOF-MS data. Using 90/10 (v/v) methanol/chloroform, tissue regions were perfectly differentiated. These results will guide future studies looking to utilize the potential of LMD-LVC/ESI-MS for tissue and disease differentiation.

Keyphrases

ms ms
mass spectrometry
multiple sclerosis
electronic health record
computed tomography
current status
ionic liquid
risk assessment
high resolution
dual energy