A ribose-functionalized NAD + with versatile activity for ADP-ribosylation.
Elisa N StephensXiao-Nan ZhangAlbert T LamJiawei LiHua PeiStan G LouieClay C C WangYong ZhangPublished in: Chemical communications (Cambridge, England) (2023)
An NAD + featuring an adenosyl 4'-azido functions as a general substrate for poly-ADP-ribose polymerases. Its derived mono- and poly-ADP-ribosylated proteins can be adequately recognized by distinct ADP-ribosylation-specific readers. This molecule represents the first ribose-functionalized NAD + with versatile activities across different ADP-ribosyltransferases and provides insight into developing new probes for ADP-ribosylation.