Spheroid formation and stemness preservation of human periodontal ligament cells on chitosan films.
Xiang-Zhen YanJjjp van den BeuckenC YuanJ A JansenF YangPublished in: Oral diseases (2018)
The results demonstrate the successful use of chitosan films for the culture of periodontal ligament cell spheroids. Compared to cells cultured in monolayer, periodontal ligament cells in spheroids did not proliferate, but exhibited higher self-renewal gene expression, colony-forming unit and osteogenic capacity.