Repurposing a DNA-Repair Enzyme for Targeted Protein Degradation.

Callie FredlenderSamantha LevineJan ZimakRobert C Spitale

Published in: Chembiochem : a European journal of chemical biology (2022)

Herein we present the exploration of the utility of DNA demethylase enzymes for targeted protein degradation. Novel benzylguanine substrates are characterized for their ability to control protein degradation in cells. Our data demonstrate the utility of this approach to degrade fusion proteins in different localizations within living cells.

Keyphrases

dna repair
living cells
protein protein
fluorescent probe
single molecule
amino acid
induced apoptosis
cancer therapy
binding protein
cell free
machine learning
drug delivery
circulating tumor
cell proliferation