Poly(ethylene glycol)-Alendronate-Coated Magnetite Nanoparticles Do Not Alter Cardiovascular Functions and Red Blood Cells' Properties in Hypertensive Rats.
Viktoriia OleksaIveta BernatovaVitalii PatsulaSilvia LiskovaPeter BališJana RadošinskáAndrea MičurováMichal KluknavskýTomáš JasenovecDominika RadošinskáHana MackováVitalii PatsulaPublished in: Nanomaterials (Basel, Switzerland) (2021)
In this study, magnetite nanoparticles were prepared and coated with poly(ethylene glycol) terminated by alendronate to ensure firm binding to the iron oxide surface. Magnetic nanoparticles, designated as magnetite coated with poly(ethylene glycol)-alendronate (Fe3O4@PEG-Ale), were characterized in terms of number-average (Dn) and hydrodynamic (Dh) size, ζ-potential, saturation magnetization, and composition. The effect of particles on blood pressure, vascular functions, nitric oxide (NO), and superoxide production in the tissues of spontaneously hypertensive rats, as well as the effect on red blood cell (RBC) parameters, was investigated after intravenous administration (1 mg Fe3O4/kg of body weight). Results showed that Fe3O4@PEG-Ale particles did negatively affect blood pressure, heart rate and RBC deformability, osmotic resistance and NO production. In addition, Fe3O4@PEG-Ale did not alter functions of the femoral arteries. Fe3O4@PEG-Ale induced increase in superoxide production in the kidney and spleen, but not in the left heart ventricle, aorta and liver. NO production was reduced only in the kidney. In conclusion, the results suggest that acute intravenous administration of Fe3O4@PEG-Ale did not produce negative effects on blood pressure regulation, vascular function, and RBCs in hypertensive rats.
Keyphrases
- red blood cell
- blood pressure
- heart rate
- drug delivery
- body weight
- nitric oxide
- heart rate variability
- hypertensive patients
- magnetic nanoparticles
- hydrogen peroxide
- high dose
- pulmonary artery
- heart failure
- gene expression
- drug induced
- iron oxide
- type diabetes
- pulmonary hypertension
- low dose
- metabolic syndrome
- blood glucose
- liver failure
- aortic dissection
- risk assessment
- mass spectrometry
- insulin resistance
- high glucose
- oxidative stress
- high resolution
- congenital heart disease
- human health
- weight loss
- endothelial cells
- hepatitis b virus