Evaluation of Allplex™ Entero-DR assay for detection of antimicrobial resistance determinants from bacterial cultures.
María Fernanda MojicaElsa De La CadenaAdriana CorreaTobías Manuel AppelChristian José PallaresMaría Virginia VillegasPublished in: BMC research notes (2020)
The method had 100% sensitivity and between 92 and 100% of specificity for identifying blaKPC, blaVIM, blaIMP, blaNDM, blaOXA-48-like, blaCTX-M and vanA. In nine isolates, unspecific amplifications were detected. The Ct of these false positives was above 33. The Ct of the correctly identified bla and van genes did not surpass 28 and 30, respectively. None of the clinical isolates included as negative controls yielded any amplification. Therefore, the Allplex™ Entero-DR assay is a highly accurate test for the detection of important antibiotic resistance determinants. With this assay, reliable results can be obtained within 3 h. However, according to our data, samples with Ct values greater than 33 should be considered with caution.
Keyphrases
- klebsiella pneumoniae
- antimicrobial resistance
- image quality
- dual energy
- computed tomography
- contrast enhanced
- high throughput
- label free
- multidrug resistant
- positron emission tomography
- loop mediated isothermal amplification
- magnetic resonance imaging
- escherichia coli
- real time pcr
- acinetobacter baumannii
- magnetic resonance
- gene expression
- electronic health record
- big data
- deep learning
- machine learning
- mass spectrometry