Aptamer-Modified Au Nanoparticles: Functional Nanozyme Bioreactors for Cascaded Catalysis and Catalysts for Chemodynamic Treatment of Cancer Cells.
Yu OuyangMichael FadeevPu ZhangRaanan CarmieliJiang LiYang Sung SohnOla KarmiRachel NechushtaiEli PikarskyChun-Hai FanItamar WillnerPublished in: ACS nano (2022)
Polyadenine-stabilized Au nanoparticles (pA-AuNPs) reveal dual nanozyme catalytic activities toward the H 2 O 2 -mediated oxidation of dopamine to aminochrome and toward the aerobic oxidation of glucose to gluconic acid and H 2 O 2 . The conjugation of a dopamine-binding aptamer (DBA) to the pA-AuNPs yields aptananozyme structures catalyzing simultaneously the H 2 O 2 -mediated oxidation of dopamine to aminochrome through the aerobic oxidation of glucose. A set of aptananozymes consisting of DBA conjugated through the 5'- or 3'-end directly or spacer bridges to pA-AuNPs were synthesized. The set of aptananozymes revealed enhanced catalytic activities toward the H 2 O 2 -catalyzed oxidation of dopamine to dopachrome, as compared to the separated pA-AuNPs and DBA constituents, and structure-function relationships within the series of aptananozymes were demonstrated. The enhanced catalytic function of the aptananozymes was attributed to the concentration of the dopamine at the catalytic interfaces by means of aptamer-dopamine complexes. The dual catalytic activities of aptananozymes were further applied to design bioreactors catalyzing the effective aerobic oxidation of dopamine in the presence of glucose. Mechanistic studies demonstrated that the aptananozymes generate reactive oxygen species. Accordingly, the AS1411 aptamer, recognizing the nucleolin receptor associated with cancer cells, was conjugated to the pA-AuNPs, yielding a nanozyme for the chemodynamic treatment of cancer cells. The AS1411 aptamer targets the aptananozyme to the cancer cells and facilitates the selective permeation of the nanozyme into the cells. Selective cytotoxicity toward MDA-MB-231 breast cancer cells ( ca . 70% cell death) as compared to MCF-10A epithelial cells ( ca . 2% cell death) is demonstrated.
Keyphrases
- sensitive detection
- uric acid
- cell death
- breast cancer cells
- hydrogen peroxide
- gold nanoparticles
- cell cycle arrest
- visible light
- prefrontal cortex
- magnetic nanoparticles
- reactive oxygen species
- high intensity
- crystal structure
- electron transfer
- metabolic syndrome
- single cell
- type diabetes
- gene expression
- wastewater treatment
- nitric oxide
- transcription factor
- reduced graphene oxide
- blood pressure
- combination therapy
- mass spectrometry
- binding protein
- ionic liquid