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Engineering DNAzyme cascade for signal transduction and amplification.

Jing LiKe QuanYanjing YangXiaohai YangXiangxian MengJin HuangKemin Wang
Published in: The Analyst (2020)
Inspired by the natural enzyme cascade reaction, a multiple DNAzyme cascade platform is engineered to imitate the intracellular process of signal transduction and signal amplification. In this design, when particular stimuli appear, an activated upstream DNAzyme will cleave a well-designed intermediary S1, releasing a downstream DNAzyme that can cleave the reporter substrate S2 to output signals. Thus, the signal is passed from the upstream DNAzyme to the downstream DNAzyme through a well-designed intermediary, accomplishing signal transduction and signal amplification. According to the experimental results, the DNAzyme cascades are capable of improving sensitivity for bioassays compared with that for single DNAzyme-based biocatalysis, which holds promise for potential applications, such as biomolecular computing, logic circuits and precision medicine.
Keyphrases
  • label free
  • living cells
  • fluorescent probe
  • single molecule
  • nucleic acid
  • crispr cas
  • single cell
  • amino acid
  • structural basis