Conjugated/nonconjugated alternating copolymers for enhanced NIR-II fluorescence imaging and NIR-II photothermal-ferrotherapy.

Conjugated polymers hold great promise for NIR-II fluorescence imaging (FI)-guided NIR-II photothermal therapy (PTT) due to the advantages of easy modification of chemical structures and adjustable NIR absorption. However, to make use of these advantages, it is of paramount importance to formulate conjugated polymers with excellent solubility in organic solution, great NIR-II photothermal conversion efficiency, and high NIR-II fluorescence quantum yield. Herein, a new class of conjugated/nonconjugated alternating copolymers (CNACPs) is reported by introducing nonconjugated linkers into a conjugated backbone to modulate the extinction coefficient at 1064 nm and NIR-II fluorescence quantum yield. The NIR-II absorption, NIR-II emission, and NIR-II photothermal properties of the new CNACPs were studied. Interestingly, it is observed that longer nonconjugated linkers in CNACPs result in higher NIR-II fluorescence intensity with sufficient NIR-II absorption and NIR-II photothermal ability. With these newly developed CNACPs (BBT-C6), phototheranostic nanoparticles (BBTD6/Fe@PMA) are prepared through facile nanoprecipitation using PMA-AD-PEG as an iron ion chelator for NIR-II FI-guided NIR-II PTT/ferrotherapy synergistic therapy. In vitro and in vivo , BBTD6/Fe@PMA effectively inhibited 4T1 cells and tumor progression under 1064 nm laser irradiation. Consequently, this work provides new CNACPs by incorporating nonconjugated linkers into a conjugated backbone to design more effective NIR-II fluorescence imaging and NIR-II photothermal therapy agents.