Detection and structural analysis of pyrimidine-derived radicals generated on DNA using a profluorescent nitroxide probe.
Kosho YamauchiYuta MatsuokaMasatomo TakahashiYoshihiro IzumiHideto NakaYosuke TaniguchiKazuaki KawaiTakeshi BambaKen-Ichi YamadaPublished in: Chemical communications (Cambridge, England) (2021)
The oxidative damage of DNA is associated with aging and the development of various diseases. Although nucleoside-derived radicals play an important role in DNA oxidation, their analysis methods are limited. Herein, we propose a fluorometric detection and structural analysis of radicals on the surface of oxidatively damaged DNA using a profluorescent nitroxide probe combined with liquid chromatography-fluorometry and high-resolution tandem mass spectrometry.
Keyphrases
- tandem mass spectrometry
- liquid chromatography
- circulating tumor
- cell free
- ultra high performance liquid chromatography
- high resolution
- single molecule
- mass spectrometry
- high performance liquid chromatography
- simultaneous determination
- gas chromatography
- high resolution mass spectrometry
- living cells
- quantum dots
- solid phase extraction
- loop mediated isothermal amplification
- nitric oxide
- sensitive detection