Detection of Few Hydrogen Peroxide Molecules Using Self-Reporting Fluorescent Nanodiamond Quantum Sensors.
Yingke WuPriyadharshini BalasubramanianZhenyu WangJaime A S CoelhoMateja PrsljaReiner SiebertMartin B PlenioFedor JelezkoTanja WeilPublished in: Journal of the American Chemical Society (2022)
Hydrogen peroxide (H 2 O 2 ) plays an important role in various signal transduction pathways and regulates important cellular processes. However, monitoring and quantitatively assessing the distribution of H 2 O 2 molecules inside living cells requires a nanoscale sensor with molecular-level sensitivity. Herein, we show the first demonstration of sub-10 nm-sized fluorescent nanodiamonds (NDs) as catalysts for the decomposition of H 2 O 2 and the production of radical intermediates at the nanoscale. Furthermore, the nitrogen-vacancy quantum sensors inside the NDs are employed to quantify the aforementioned radicals. We believe that our method of combining the peroxidase-mimicking activities of the NDs with their intrinsic quantum sensor showcases their application as self-reporting H 2 O 2 sensors with molecular-level sensitivity and nanoscale spatial resolution. Given the robustness and the specificity of the sensor, our results promise a new platform for elucidating the role of H 2 O 2 at the cellular level.
Keyphrases
- hydrogen peroxide
- living cells
- single molecule
- atomic force microscopy
- fluorescent probe
- nitric oxide
- molecular dynamics
- low cost
- quantum dots
- energy transfer
- adverse drug
- photodynamic therapy
- monte carlo
- high throughput
- emergency department
- highly efficient
- high resolution
- loop mediated isothermal amplification
- deep learning