Rapid Prototyping of 3D-Printed AgNPs- and Nano-TiO 2 -Embedded Hydrogels as Novel Devices with Multiresponsive Antimicrobial Capability in Wound Healing.
Giulia RemaggiLaura BergamontiClaudia GraiffMaria Cristina OssiprandiLisa ElviriPublished in: Antibiotics (Basel, Switzerland) (2023)
Two antimicrobial agents such as silver nanoparticles (AgNPs) and titanium dioxide (TiO 2 ) have been formulated with natural polysaccharides (chitosan or alginate) to develop innovative inks for the rapid, customizable, and extremely accurate manufacturing of 3D-printed scaffolds useful as dressings in the treatment of infected skin wounds. Suitable chemical-physical properties for the applicability of these innovative devices were demonstrated through the evaluation of water content (88-93%), mechanical strength (Young's modulus 0.23-0.6 MPa), elasticity, and morphology. The antimicrobial tests performed against Staphylococcus aureus and Pseudomonas aeruginosa demonstrated the antimicrobial activities against Gram+ and Gram- bacteria of AgNPs and TiO 2 agents embedded in the chitosan (CH) or alginate (ALG) macroporous 3D hydrogels (AgNPs MIC starting from 5 µg/mL). The biocompatibility of chitosan was widely demonstrated using cell viability tests and was higher than that observed for alginate. Constructs containing AgNPs at 10 µg/mL concentration level did not significantly alter cell viability as well as the presence of titanium dioxide; cytotoxicity towards human fibroblasts was observed starting with an AgNPs concentration of 100 µg/mL. In conclusions, the 3D-printed dressings developed here are cheap, highly defined, easy to manufacture and further apply in personalized antimicrobial medicine applications.
Keyphrases
- wound healing
- silver nanoparticles
- staphylococcus aureus
- drug delivery
- biofilm formation
- pseudomonas aeruginosa
- tissue engineering
- quantum dots
- hyaluronic acid
- gram negative
- endothelial cells
- mental health
- cystic fibrosis
- physical activity
- visible light
- extracellular matrix
- escherichia coli
- induced pluripotent stem cells
- drug resistant
- middle aged
- drug release
- acinetobacter baumannii
- replacement therapy