Visual Monitoring of Nucleic Acid Dynamic Structures during Cellular Ferroptosis Using Rationally Designed Carbon Dots with Robust Anti-Interference Ability to Reactive Oxygen Species.

Ferroptosis triggered by an iron-dependent accumulation of lipid reactive oxygen species (ROS) has drawn widespread attention. Directly visualizing the dynamic structures of nucleic acids during the ferroptosis of cells is of great importance considering their vital roles in numerous biological functions. However, direct imaging remains challenging, largely due to the extremely high concentrations of ROS generated during ferroptosis, which can affect the imaging of nucleic acid targeted fluorescent probes. To overcome this challenge, nucleic acid-responsive carbon dots (CDs) providing favorable optical properties together with high chemical stability were synthesized. Furthermore, the CDs penetrated the cell membrane quickly and accumulated in the nuclei of cells. The robust anti-interference ability to ROS allows the CDs to visualize the dynamic structures of nucleic acids during ferroptosis. Moreover, the CDs were successfully employed in the imaging of nucleic acids during cell division. The nuclei-targeting CDs show great potential as a powerful tool for imaging nuclei in ferroptosis-related biological and clinical research.