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Light-sensing via hydrogen peroxide and a peroxiredoxin.

Kristofer BodvardKen PeetersFriederike RogerNatalie RomanovAeid IgbariaNiek WelkenhuysenGaël PalaisWolfgang ReiterMichel B ToledanoMikael KällMikael Molin
Published in: Nature communications (2017)
Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H2O2) signal that is sensed by the peroxiredoxin Tsa1 and transduced to thioredoxin, to counteract PKA-dependent Msn2 phosphorylation. Upon H2O2, the nuclear retention of PKA catalytic subunits, which contributes to delayed Msn2 nuclear concentration, is antagonized in a Tsa1-dependent manner. Conversely, peroxiredoxin hyperoxidation interrupts the H2O2 signal and drives Msn2 oscillations by superimposing on PKA feedback regulation. Our data identify a mechanism by which light could be sensed in all cells lacking dedicated photoreceptors. In particular, the use of H2O2 as a second messenger in signalling is common to Msn2 oscillations and to light-induced entrainment of circadian rhythms and suggests conserved roles for peroxiredoxins in endogenous rhythms.
Keyphrases
  • hydrogen peroxide
  • transcription factor
  • nitric oxide
  • working memory
  • induced apoptosis
  • big data
  • signaling pathway
  • genome wide identification