Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry: A New Tool Complementing Metabolomic Analyses of Polar Metabolites.
Jun ZengZhi-Chao WangXin HuangSabine S EcksteinXiaohui LinHailong PiaoCora WeigertPeiyuan YinRainer LehmannGuo-Wang XuPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2019)
Mass spectrometry (MS) driven metabolomics is a frequently used tool in various areas of life sciences; however, the analysis of polar metabolites is less commonly included. In general, metabolomic analyses lead to the detection of the total amount of all covered metabolites. This is currently a major limitation with respect to metabolites showing high turnover rates, but no changes in their concentration. Such metabolites and pathways could be crucial metabolic nodes (e.g., potential drug targets in cancer metabolism). A stable-isotope tracing capillary electrophoresis-mass spectrometry (CE-MS) metabolomic approach was developed to cover both polar metabolites and isotopologues in a non-targeted way. An in-house developed software enables high throughput processing of complex multidimensional data. The practicability is demonstrated analyzing [U-13 C]-glucose exposed prostate cancer and non-cancer cells. This CE-MS-driven analytical strategy complements polar metabolite profiles through isotopologue labeling patterns, thereby improving not only the metabolomic coverage, but also the understanding of metabolism.
Keyphrases
- mass spectrometry
- capillary electrophoresis
- ms ms
- liquid chromatography
- prostate cancer
- gas chromatography
- high performance liquid chromatography
- high resolution
- high throughput
- ionic liquid
- young adults
- cancer therapy
- emergency department
- type diabetes
- multiple sclerosis
- radiation therapy
- blood glucose
- metabolic syndrome
- big data
- artificial intelligence
- early stage
- human health
- healthcare
- bone mineral density
- childhood cancer
- lymph node
- solid phase extraction
- climate change
- rectal cancer