Self-Assembled Nanocomposite Hydrogels as Carriers for Demineralized Bone Matrix Particles and Enhanced Bone Repair.
Chen ChenZhi LiChanglu XuMinjee KangChung-Sung LeeTara AghalooMin LeePublished in: Advanced healthcare materials (2024)
Demineralized bone matrix (DBM) has been widely used as an allogeneic alternative to autologous bone graft for bone repair. However, more extensive use of DBM is limited due to its particulate nature after demineralization and rapid particle dispersion following irrigation, resulting in unpredictable osteoinductivity. Here, a new design of injectable hydrogel carriers for DBM that combine self-healing ability and osteogenic properties based on the self-assembly of guanidinylated hyaluronic acid and silica-rich nanoclays is reported. The nanoclays serve as reversible linkages to form a dynamic hydrogel network with the guanidine moieties on the polymer chains. Gelation kinetics and mechanical properties can be controlled by altering nanoclay content in the hydrogel. The resulting hydrogel exerts self-healing ability due to its dynamic crosslinks and well retains its overall performance with high DBM loading. The hydrogel exhibits great cytocompatibility and osteogenic effects mediated by the nanoclays. In vivo delivery of DBM using the nanocomposite hydrogel further demonstrates robust bone regeneration in a mouse calvarial defect model in comparison to DBM delivered with aqueous HA. This work suggests a promising hydrogel platform for many applications including therapeutic delivery and tissue engineering.
Keyphrases
- hyaluronic acid
- tissue engineering
- bone regeneration
- drug delivery
- bone mineral density
- bone marrow
- mesenchymal stem cells
- wound healing
- soft tissue
- bone loss
- postmenopausal women
- stem cell transplantation
- high throughput
- stem cells
- high dose
- body composition
- aqueous solution
- sensitive detection
- highly efficient
- stress induced
- reduced graphene oxide
- single cell
- high resolution