Ratiometric Fluorescent Detection of Intracellular Singlet Oxygen by Semiconducting Polymer Dots.
Weiying HouYe YuanZezhou SunShuxu GuoHaowen DongChangfeng WuPublished in: Analytical chemistry (2018)
Singlet oxygen (1O2) plays important roles in many biological processes. However, it is very difficult to detect 1O2 in the intracellular environment because of its relatively low concentration and short lifetime. Here, we developed a ratiometric probe based on semiconducting polymer dots (Pdots) that can sensitively detect 1O2 in live cells. An organic dye, singlet oxygen sensor green (SOSG), was doped in polyfluorene Pdots, and excitation energy was efficiently transferred from the polymer to the SOSG dye. Accordingly, the Pdots showed constant blue fluorescence as a reference, and increased green fluorescence upon singlet oxygen generation. The ratiometric response of Pdots was examined in the intracellular environment by in situ 1O2 generation with a photosensitizer and light irradiation. Both spectroscopic measurements and confocal imaging were performed to monitor intracellular 1O2 generation during photodynamic therapy using the Pdot probe. Our results indicate that the SOSG-doped Pdots are promising for intracellular 1O2 detection.
Keyphrases
- quantum dots
- energy transfer
- living cells
- fluorescent probe
- photodynamic therapy
- sensitive detection
- reactive oxygen species
- single molecule
- highly efficient
- loop mediated isothermal amplification
- label free
- induced apoptosis
- real time pcr
- fluorescence imaging
- high resolution
- molecular docking
- cell death
- cell proliferation
- oxidative stress
- cell cycle arrest
- radiation induced
- optical coherence tomography
- molecularly imprinted
- liquid chromatography
- metal organic framework
- light emitting