Simultaneous determination and anti-inflammatory effects of four phenolic compounds in Dendrobii Herba.
Sae-Rom YooSoo-Jin JeongNa-Ri LeeHyeun-Kyoo ShinChang-Seob SeoPublished in: Natural product research (2017)
Dendrobii Herba, a traditional Korean medicine, is used for treating atrophic gastritis, diabetes and cardiovascular diseases. Phytochemical studies of Dendrobium species and their compounds have been conducted. However, the pharmaceutical effects of these compounds have not yet been elucidated. We performed quantitative determination of four phenolic compounds, - (1) 4-hydroxybenzoic acid, (2) vanillic acid (3) syringic acid and (4) ferulic acid - in Dendrobii Herba using high-performance liquid chromatography coupled with a photodiode array detector. In addition, we investigated the effects of compounds in LPS-stimulated RAW 264.7 cells by measuring of inflammatory mediators. Among the four compounds, 1-3 had a significant inhibitory effect on TNF-α production. The levels of IL-6 were significantly reduced by treatment with compounds 13 and 4 compared with LPS treated cell. All compounds significantly reduced LPS-stimulated PGE2 production. Thus, these four marker compounds from Dendrobii Herba exhibit anti-inflammatory activity by targeting different inflammation-related cytokines.
Keyphrases
- high performance liquid chromatography
- simultaneous determination
- solid phase extraction
- cardiovascular disease
- tandem mass spectrometry
- inflammatory response
- type diabetes
- oxidative stress
- liquid chromatography tandem mass spectrometry
- computed tomography
- rheumatoid arthritis
- helicobacter pylori
- single cell
- induced apoptosis
- high throughput
- high resolution
- coronary artery disease
- bone marrow
- cell therapy
- helicobacter pylori infection
- mesenchymal stem cells
- combination therapy
- skeletal muscle
- cell death
- contrast enhanced
- pi k akt
- newly diagnosed