Surface Structuring Combined with Chemical Surface Functionalization: An Effective Tool to Manipulate Cell Adhesion.
Sarah M ElsayedStefan PaschkeSibylle J RauKaren LienkampPublished in: Molecules (Basel, Switzerland) (2019)
In this study, we investigate how a surface structure underneath a surface-attached polymer coating affects the bioactivity of the resulting material. To that end, structured surfaces were fabricated using colloidal lithography (lateral dimensions: 200 nm to 1 µm, height ~15 to 50 nm). The surface structures were further functionalized either with antimicrobial, cell-adhesive polycations or with protein-repellent polyzwitterions. The materials thus obtained were compared to non-functionalized structured surfaces and unstructured polymer monolayers. Their physical properties were studied by contact-angle measurements and atomic force microscopy (AFM). Protein adhesion was studied by surface plasmon resonance spectroscopy, and the antimicrobial activity against Escherichia coli bacteria was tested. The growth of human mucosal gingiva keratinocytes on the materials was analyzed using the Alamar blue assay, optical microscopy, and live-dead staining. The data shows that the underlying surface structure itself reduced protein adhesion and also bacterial adhesion, as evidenced by increased antimicrobial activity. It also enhanced cell adhesion to the surfaces. Particularly in combination with the adhesive polycations, the surfaces increased the cell growth compared to the unstructured reference materials. Thus, functionalizing structured surfaces with adhesive polymer could be a valuable tool for improved tissue integration.
Keyphrases
- cell adhesion
- biofilm formation
- high resolution
- atomic force microscopy
- escherichia coli
- high speed
- staphylococcus aureus
- single molecule
- physical activity
- endothelial cells
- high throughput
- mental health
- protein protein
- binding protein
- body mass index
- candida albicans
- photodynamic therapy
- minimally invasive
- quantum dots
- mesenchymal stem cells
- electronic health record
- machine learning
- mass spectrometry
- big data
- molecularly imprinted
- wound healing
- ulcerative colitis
- transition metal