Decontamination, pooling and dereplication of the 678 samples of the Marine Microbial Eukaryote Transcriptome Sequencing Project.

Mick Van VlierbergheArnaud Di FrancoHervé PhilippeDenis Baurain

Published in: BMC research notes (2021)

We submitted 678 MMETSP re-assembly samples to our parallel consolidation pipeline. Hence, we combined 423 samples into 110 consolidated transcriptomes, after the systematic removal of the most contaminated samples (186). This approach resulted in a total of 224 high-quality transcriptomes, easy to use and suitable to compute less contaminated, less redundant and more balanced phylogenies.

Keyphrases

single cell
heavy metals
drinking water
rna seq
gene expression
quality improvement
microbial community
risk assessment
dna methylation