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Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering.
Shuaili Chen
Zhanhao Yang
Ze Zhong
Shiqin Yu
Jingwen Zhou
Jianghua Li
Guocheng Du
Guoqiang Zhang
Published in:
Biotechnology for biofuels and bioproducts (2024)
In this study, thermal-responsive targeted mutagenesis coupled with ultrahigh-throughput screening was developed for the rapid evolution of enzymes and biosynthetic pathways.
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