Anticancer effects of Artemisia campestris extract on acute myeloid leukemia cells: an ex vivo study.
Rachid KharratFatma Ben LakhalHiba SouiaInes LimamHend Ben NajiMohamed AbdelkarimPublished in: Medical oncology (Northwood, London, England) (2024)
Cure rates for acute myeloid leukemia (AML) remain suboptimal; thus, new treatment strategies are needed for this deadly disease. Artemisia campestris leaves hold significant value in traditional medicine. Despite extensive research conducted on this plant globally, the specific anti-AML properties of the leaves have received limited investigation. This study aims to explore the potential anti-leukemic activities of the ethyl acetate extract derived from Artemisia campestris (EAEAC), using mononuclear cells from bone marrow of thirteen AML patients. To this end, cytotoxic effects were evaluated using the MTT assay, and the mechanisms of cell death were investigated through various methods, including propidium iodide staining, annexin V/propidium iodide double staining, mitochondrial depolarization, and caspase-3/7 activation assays. Results demonstrated that EAEAC induced cell apoptosis by increasing DNA fragmentation, causing mitochondrial depolarization, and activating caspases 3/7. On the other hand, we assessed EAEAC's effect on two leukemia stem cell subpopulations, with results suggesting a potential decrease in their frequencies (three/five patients).
Keyphrases
- acute myeloid leukemia
- cell death
- end stage renal disease
- bone marrow
- oxidative stress
- stem cells
- allogeneic hematopoietic stem cell transplantation
- chronic kidney disease
- ejection fraction
- newly diagnosed
- induced apoptosis
- prognostic factors
- high throughput
- mesenchymal stem cells
- high resolution
- acute lymphoblastic leukemia
- endoplasmic reticulum stress
- cell free
- atomic force microscopy
- essential oil
- cell therapy
- high glucose
- high speed