Evaluation of a New Closed-System Automated RT-qPCR Assay for the Rapid Detection and Monitoring of Common Nucleophosmin Mutations in Patients with Acute Myeloid Leukemia.
Richard D PressDana DresselMichelle McBeanIng Soo TiongMatthew W AndersonDavid PrideAarthi RamanRachelle R DoomRajesh KaldatePublished in: International journal of molecular sciences (2024)
Quantitative assessment of nucleophosmin 1 ( NPM1 ) mutation status is integral to evaluating measurable residual disease (MRD) in NPM1 -mutated acute myeloid leukemia (AML) patients. In a retrospective study, leftover peripheral blood (PB) specimens (n = 40) which were collected for routine clinical diagnostic evaluations of AML disease burden were tested by both a novel automated RT-qPCR quantitative NPM1 assay (Xpert NPM1 mutation assay) and the NPM1 mutA, mutB&D MutaQuant kit. Based on a Deming regression analysis, there was a high correlation (slope = 0.92; intercept = 0.12; Pearson's r = 0.982) between the quantitative results of the Xpert NPM1 mutation assay and the NPM1 mutA, mutB&D MutaQuant kit. The Xpert test quantitative results are thus highly correlated with the comparator method and the former has potential as a useful alternative for the monitoring of AML patients with a known NPM1 mutation.
Keyphrases
- acute myeloid leukemia
- high throughput
- allogeneic hematopoietic stem cell transplantation
- peripheral blood
- high resolution
- machine learning
- deep learning
- ejection fraction
- end stage renal disease
- newly diagnosed
- loop mediated isothermal amplification
- risk assessment
- mass spectrometry
- human health
- single cell
- quantum dots
- sensitive detection