UV/H 2 O 2 -Degraded Polysaccharides from Sargassum fusiforme : Purification, Structural Properties, and Anti-Inflammatory Activity.

The main purpose of this study was to analyze the structural properties and anti-inflammatory activity of the purified fractions derived from UV/H 2 O 2 -degraded polysaccharides from Sargassum fusiforme . Results indicated that twofractions with different monosaccharide compositions and morphological characteristics, PT-0.25 (yield 39.5%) and PT-0.5 (yield 23.9%), were obtained. The average molecular weights of PT-0.25 and PT-0.5 were 14.52 kDa and 22.89 kDa, respectively. In addition, PT-0.5 exhibited better anti-inflammatory activity with a clear dose dependence. The mechanism was associated with the inhibition of LPS-activated Toll-like receptor 4-mediated inflammatory pathways in RAW264.7 cells. The results showed that PT-0.5 was a complex polysaccharide mainly composed of 4-Fuc p , t-Man p , 6-Gal p , t-Fuc p , and 3,4-GlcA p . These results would provide theoretical support for studying the structural properties and biological activities of UV/H 2 O 2 -degraded polysaccharides.