Peptide-fluorophore/AuNP conjugate-based two-photon excited fluorescent nanosensor for caspase-3 activity imaging assay in living cells and tissue.
Qingshan GeNingning WangJishan LiRonghua YangPublished in: MedChemComm (2017)
In the present study, a novel fluorescence resonance energy transfer (FRET) strategy-based two-photon excited nanosensor via the assembly of two-photon dye-labeled peptides on the gold nanoparticle's surface has been developed and successfully applied in the caspase-3 imaging assay in living cells and ischemia-reperfusion surgery-treated rat liver tissue slices.
Keyphrases
- living cells
- energy transfer
- fluorescent probe
- single molecule
- quantum dots
- high resolution
- cell death
- high throughput
- minimally invasive
- induced apoptosis
- oxidative stress
- pet imaging
- amino acid
- endoplasmic reticulum stress
- acute coronary syndrome
- cancer therapy
- surgical site infection
- atrial fibrillation
- label free