Stimuli-Responsive Microfluidic Interface Enables Highly Efficient Capture and Release of Circulating Fetal Cells for Non-Invasive Prenatal Testing.
Huimin ZhangYuanyuan YangYilong LiuYidi WangWeidong RuanJia SongXiyuan YuLingling WuZhi ZhuGuolin HongChaoyong James YangPublished in: Analytical chemistry (2020)
Circulating fetal nucleated cells (CFCs) carrying whole genomic coding of the fetus in maternal blood have been pursued as ideal biomarkers for noninvasive prenatal testing (NIPT). However, a significant limitation is the need to enrich sufficient cells in quantity and purity for fetal genetic disorder diagnosis. This study for the first time demonstrates a stimuli-responsive ligand enabling interface on array patterned microfluidic chip (NIPT-Chip) for high efficient isolation and release of CFCs in untreated whole blood. Deterministic lateral displacement (DLD)-array was patterned in the chip to increase collision frequency between CFCs and surface-anchored antibody to achieve high efficient cell capture. More importantly, the stimuli-responsive interface enables gentle release of captured CFCs through a thiol exchange reaction for downstream gene analysis of NIPT. With the advantages of simple processing, efficient isolation, and gentle release, NIPT-Chip offers great potential for clinical translation of circulating fetal cell-based NIPT.
Keyphrases
- high throughput
- circulating tumor cells
- induced apoptosis
- single cell
- cell cycle arrest
- highly efficient
- pregnant women
- copy number
- genome wide
- risk assessment
- oxidative stress
- high resolution
- signaling pathway
- gene expression
- minimally invasive
- cell death
- transcription factor
- drug delivery
- dna methylation
- mass spectrometry
- body mass index
- climate change
- birth weight
- weight gain
- preterm birth