Single-cell RNA sequencing of mitotic-arrested prospermatogonia with DAZL::GFP chickens and revealing unique epigenetic reprogramming of chickens.

Hyeon Jeong ChoiKyung Min JungDeivendran RengarajKyung Youn LeeEunhui YooTae Hyun KimJae-Yong Han

Published in: Journal of animal science and biotechnology (2022)

We found a unique epigenetic-reprogramming schedule of mitotic-arrested chicken prospermatogonia and prolonged hypomethylation after hatching. This will provide a foundation for understanding the process of germ-cell epigenetic regulation in several species for which this process is not clearly described. Our findings on the biological processes related to sex-specific differentiation of prospermatogonia could help studying germline development in vitro more elaborately.

Keyphrases

single cell
germ cell
dna methylation
rna seq
cell cycle
heat stress
gene expression
high throughput
disease virus
dna repair
cell proliferation