Altered metabolism of chloroplastic NAD kinase-overexpressing Arabidopsis in response to magnesium sulfate supplementation.
Maki Kawai-YamadaAtsuko MiyagiYuki SatoYuki HosoiShin-Nosuke HashidaToshiki IshikawaMasatoshi YamaguchiPublished in: Plant signaling & behavior (2020)
Nicotinamide adenine dinucleotide (NAD)/NAD phosphate (NADPH) is essential for numerous redox reactions and serve as co-factors in multiple metabolic processes in all organisms. NAD kinase (NADK) is an enzyme involved in the synthesis of NADP+ from NAD+ and ATP. Arabidopsis NADK2 (AtNADK2) is a chloroplast-localizing enzyme that provides recipients of reducing power in photosynthetic electron transfer. When Arabidopsis plants were grown on MS medium supplemented with 5 mM MgSO4, an AtNADK2-overexpressing line exhibited higher glutathione and total sulfur accumulation than control plants. Metabolomic analysis of major amino acids and organic acids using capillary electrophoresis-mass spectrometry demonstrated that overexpression of AtNADK2 affected a range of metabolic processes in response to MgSO4 supplementation.
Keyphrases
- mass spectrometry
- capillary electrophoresis
- transcription factor
- electron transfer
- liquid chromatography
- plant growth
- amino acid
- multiple sclerosis
- gas chromatography
- cell wall
- cell proliferation
- ms ms
- high performance liquid chromatography
- high resolution
- multidrug resistant
- reactive oxygen species
- kidney transplantation