Metabolic and Molecular Rearrangements of Sauvignon Blanc ( Vitis vinifera L.) Berries in Response to Foliar Applications of Specific Dry Yeast.
Marta RodriguesCristian ForestanLaura RavazzoloPhilippe HugueneyRaymonde BaltenweckAngela RasoriValerio CardilloPietro CarraroMario MalagoliStefano BrizzolaraSilvia QuaggiottiDuilio PorroFranco MeggioClaudio BonghiFabrizio BattistaBenedetto RupertiPublished in: Plants (Basel, Switzerland) (2023)
Dry yeast extracts (DYE) are applied to vineyards to improve aromatic and secondary metabolic compound content and wine quality; however, systematic information on the underpinning molecular mechanisms is lacking. This work aimed to unravel, through a systematic approach, the metabolic and molecular responses of Sauvignon Blanc berries to DYE treatments. To accomplish this, DYE spraying was performed in a commercial vineyard for two consecutive years. Berries were sampled at several time points after the treatment, and grapes were analyzed for sugars, acidity, free and bound aroma precursors, amino acids, and targeted and untargeted RNA-Seq transcriptional profiles. The results obtained indicated that the DYE treatment did not interfere with the technological ripening parameters of sugars and acidity. Some aroma precursors, including cys-3MH and GSH-3MH, responsible for the typical aromatic nuances of Sauvignon Blanc, were stimulated by the treatment during both vintages. The levels of amino acids and the global RNA-seq transcriptional profiles indicated that DYE spraying upregulated ROS homeostatic and thermotolerance genes, as well as ethylene and jasmonic acid biosynthetic genes, and activated abiotic and biotic stress responses. Overall, the data suggested that the DYE reduced berry oxidative stress through the regulation of specific subsets of metabolic and hormonal pathways.
Keyphrases
- rna seq
- single cell
- amino acid
- oxidative stress
- highly efficient
- gene expression
- genome wide
- type diabetes
- dna damage
- mass spectrometry
- transcription factor
- cell death
- heat shock
- metabolic syndrome
- insulin resistance
- drug delivery
- adipose tissue
- saccharomyces cerevisiae
- polycystic ovary syndrome
- reactive oxygen species
- fluorescent probe
- replacement therapy
- heat shock protein
- smoking cessation