Recently CuO nanoparticles (n-CuO) have been proposed as an alternative method to deliver a Cu-based pesticide for controlling fungal infestations. With the concomitant use of glyphosate as an herbicide, the interactions between n-CuO and this strong ligand need to be assessed. We investigated the dissolution kinetics of n-CuO and bulk-CuO (b-CuO) particles in the presence of a commercial glyphosate product and compared it to oxalate, a natural ligand present in soil water. We performed experiments at concentration levels representative of the conditions under which n-CuO and glyphosate would be used (∼0.9 mg/L n-CuO and 50 μM of glyphosate). As tenorite (CuO) dissolution kinetics are known to be surface controlled, we determined that at pH 6.5, T ∼ 20 °C, using KNO 3 as background electrolyte, the presence of glyphosate leads to a dissolution rate of 9.3 ± 0.7 ×10 -3 h -1 . In contrast, in absence of glyphosate, and under the same conditions, it is 2 orders of magnitude less: 8.9 ± 3.6 ×10 -5 h -1 . In a more complex multi-electrolyte aqueous solution the same effect is observed; glyphosate promotes the dissolution rates of n-CuO and b-CuO within the first 10 h of reaction by a factor of ∼2 to ∼15. In the simple KNO 3 electrolyte, oxalate leads to dissolution rates of CuO about two times faster than glyphosate. However, the kinetic rates within the first 10 h of reaction are about the same for the two ligands when the reaction takes place in the multi-electrolyte solution as oxalate is mostly bound to Ca 2+ and Mg 2+ .