Pseudobulk with proper offsets has the same statistical properties as generalized linear mixed models in single-cell case-control studies.

Published in: Bioinformatics (Oxford, England) (2024)

Offset pseudobulk can be easily implemented in any generalized linear model software by tweaking a few options. The codes can be found at https://github.com/hanbin973/pseudobulk_is_mm.

Keyphrases

case control
single cell
rna seq
high throughput
data analysis