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Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA.

Carolina SenraLuciana Inácia GomesLiliane Maria Vidal SiqueiraPaulo Marcos Zech CoelhoAna RabelloEdward José de Oliveira
Published in: BMC research notes (2018)
The PCR-ELISA laboratorial platform indicated a prevalence rate of 25.2%, which was higher than the Kato-Katz technique (18.4%) and lower than the commercial platform (30.1%). Considering Kato-Katz technique as the reference, there were 97.4% and 91.1% of relative sensitivity and specificity rates, respectively. The laboratorial platform presented good precision, performance diagnostic, and can be used in replacement to the commercial platform for diagnosis of schistosomiasis by PCR-ELISA.
Keyphrases
  • high throughput
  • monoclonal antibody