Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides.

Tucker J CarrocciLea LoheMatthew J AshtonClaudia Höbartner Aaron A Hoskins

Published in: Chemical communications (Cambridge, England) (2017)

The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2',5'-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTPS were found to be resistant to debranchase.

Keyphrases

quantum dots
living cells
fluorescent probe
label free
glycemic control
gene expression
structure activity relationship
pet imaging
cancer therapy
metabolic syndrome
drug delivery
single molecule
dna binding
adipose tissue
hiv testing
transcription factor