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A new genotype of border disease virus with implications for molecular diagnostics.

Simone PelettoClaudio CarusoFrancesco CeruttiPaola ModestoSimona ZoppiAlessandro DondoPier Luigi AcutisLoretta Masoero
Published in: Archives of virology (2015)
Border disease virus (BDV) is a (+) single-stranded RNA pestivirus affecting mainly sheep and goats worldwide. Genetic typing of BDV has led to the identification of at least seven major genotypes. This study reports the detection of a BDV strain from a goat in northwestern Italy during routine investigations. Sequence analysis revealed mutations in the 5'-UTR of the virus with implications for BDV molecular diagnostics. Moreover, subsequent phylogenetic analysis based on the combined 5'-UTR and Npro/partial C genes, showed divergence from known BDV genotypes, revealing the detection of a novel pestivirus group, for which we propose the name BDV genotype 8.
Keyphrases
  • disease virus
  • genome wide
  • loop mediated isothermal amplification
  • label free
  • real time pcr
  • bioinformatics analysis
  • emergency department
  • gene expression
  • single cell
  • adverse drug
  • amino acid
  • light emitting