Phytocomplex of a Standardized Extract from Red Orange ( Citrus sinensis L. Osbeck) against Photoaging.
Barbara Maria Rita TomaselloGiuseppe Antonio MalfaRosaria AcquavivaAlfonsina La MantiaClaudia Di GiacomoPublished in: Cells (2022)
Excessive exposure to solar radiation is associated with several deleterious effects on human skin. These effects vary from the occasional simple sunburn to conditions resulting from chronic exposure such as skin aging and cancers. Secondary metabolites from the plant kingdom, including phenolic compounds, show relevant photoprotective activities. In this study, we evaluated the potential photoprotective activity of a phytocomplex derived from three varieties of red orange ( Citrus sinensis (L.) Osbeck). We used an in vitro model of skin photoaging on two human cell lines, evaluating the protective effects of the phytocomplex in the pathways involved in the response to damage induced by UVA-B. The antioxidant capacity of the extract was determined at the same time as evaluating its influence on the cellular redox state (ROS levels and total thiol groups). In addition, the potential protective action against DNA damage induced by UVA-B and the effects on mRNA and protein expression of collagen, elastin, MMP1, and MMP9 were investigated, including some inflammatory markers (TNF-α, IL-6, and total and phospho NFkB) by ELISA. The obtained results highlight the capacity of the extract to protect cells both from oxidative stress-preserving RSH ( p < 0.05) content and reducing ROS ( p < 0.01) levels-and from UVA-B-induced DNA damage. Furthermore, the phytocomplex is able to counteract harmful effects through the significant downregulation of proinflammatory markers ( p < 0.05) and MMPs ( p < 0.05) and by promoting the remodeling of the extracellular matrix through collagen and elastin expression. This allows the conclusion that red orange extract, with its strong antioxidant and photoprotective properties, represents a safe and effective option to prevent photoaging caused by UVA-B exposure.
Keyphrases
- oxidative stress
- dna damage
- induced apoptosis
- diabetic rats
- extracellular matrix
- dna repair
- ischemia reperfusion injury
- wound healing
- anti inflammatory
- endothelial cells
- rheumatoid arthritis
- cell death
- soft tissue
- drug induced
- physical activity
- signaling pathway
- cell migration
- reactive oxygen species
- ms ms
- body mass index
- human health
- radiation induced
- endoplasmic reticulum stress
- weight gain
- cell cycle arrest
- monoclonal antibody
- induced pluripotent stem cells
- atomic force microscopy