Effect of small amounts of akaganeite (β-FeOOH) nanorods on the gelation, phase behaviour and injectability of thermoresponsive Pluronic F127.

Pluronic F127 (PF127) is a copolymer with an amphiphilic nature and can self-assemble to form micelles and, beyond 20% (w/v), form a thermoresponsive physical gel state. However, they are mechanically weak and easily dissolve in physiological environments, which limits their use in load-bearing in specific biomedical applications. Therefore, we propose a pluronic-based hydrogel with enhanced stability by incorporating small amounts of paramagnetic nanorods, akaganeite (β-FeOOH) nanorods (NRs) of aspect ratio ∼7, with PF127. Due to their weak magnetic properties, β-FeOOH NRs have been used as a precursor for preparing stable iron-oxide states ( e.g. , hematite and magnetite), and the studies on β-FeOOH NRs to be used as a primary component in hydrogels are at the nascent stage. Here we report a method to synthesize β-FeOOH NRs on a gram scale using a simple sol-gel process and characterize the NRs with various techniques. A phase diagram and thermoresponsive behaviour based on rheological experiments and visual observations are proposed for 20% (w/v) PF127 with low concentrations (0.1-1.0% (w/v)) of β-FeOOH NRs. We observe a unique non-monotonous behaviour in the gel network represented by various rheological parameters like storage modulus, yield stress, fragility, high-frequency modulus plateau, and characteristic relaxation time as a function of nanorod concentration. A plausible physical mechanism is proposed to fundamentally understand the observed phase behaviour in the composite gels. These gels show thermoresponsiveness and enhanced injectability, and could find applications in tissue engineering and drug delivery.