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The intestinal permeability marker FITC-dextran 4kDa should be dosed according to lean body mass in obese mice.

Louise M VoetmannBidda RolinRikke K KirkCharles PykeAxel Kornerup Hansen
Published in: Nutrition & diabetes (2023)
Evaluation of the intestinal permeability by FD-4 with the typical bodyweight dose regimen in obese mice will be confounded by the significant difference in dose given when compared to a lean control group. If the test dose is based on lean body mass, no significant difference in intestinal permeability is observed, even with large group sizes. Furthermore, we showed a dose-dependent difference in plasma FD-4 levels in lean mice. Therefore, we conclude that the dose should be based on lean body mass for the FD-4 permeability test if mice with considerable obesity differences are to be compared or to use another test with fixed doses.
Keyphrases
  • bone mineral density
  • high fat diet induced
  • endothelial cells
  • type diabetes
  • metabolic syndrome
  • insulin resistance
  • postmenopausal women
  • weight loss
  • body mass index
  • physical activity
  • weight gain