MitoRS, a method for high throughput, sensitive, and accurate detection of mitochondrial DNA heteroplasmy.
Julien MarquisGregory LefebvreYiannis A I KourmpetisMohamed KassamFrédéric RongaUmberto De MarchiAndreas WiederkehrPatrick DescombesPublished in: BMC genomics (2017)
The MitoRS method enables the robust, accurate, and sensitive analysis of a large number of samples. Because it is cost effective and simple to setup, we anticipate this method will promote the analysis of mtDNA variants in large cohorts, and may help assessing the impact of mtDNA heteroplasmy on metabolic health, brain function, cancer progression, or ageing.
Keyphrases
- mitochondrial dna
- copy number
- high throughput
- genome wide
- high resolution
- papillary thyroid
- public health
- healthcare
- dna methylation
- mental health
- white matter
- single cell
- squamous cell
- real time pcr
- young adults
- loop mediated isothermal amplification
- squamous cell carcinoma
- label free
- functional connectivity
- social media
- sensitive detection