THE ROLE OF DNA DOUBLE-STRAND BREAK REPAIR THROUGH NON-HOMOLOGOUS END JOINING IN THE DOSE-RATE EFFECT IN TERMS OF CLONOGENIC ABILITY.
Hisayo TsuchiyaMikio ShimadaKaima TsukadaQingmei MengJunya KobayashiYoshihisa MatsumotoPublished in: Radiation protection dosimetry (2022)
It is generally and widely accepted that the biological effects of a given dose of ionizing radiation, especially those of low linear energy transfer radiations like X-ray and gamma ray, become smaller as the dose rate becomes lower. This phenomenon, known as 'dose-rate effect (DRE),' is considered due to the repair of sublethal damage during irradiation but the precise mechanisms for DRE have remained to be clarified. We recently showed that DRE in terms of clonogenic cell survival is diminished or even inversed in rodent cells lacking Ku, which is one of the essential factors in the repair of DNA double-strand breaks (DSBs) through non-homologous end joining (NHEJ). Here we review and discuss the involvement of NHEJ in DRE, which has potential implications in radiological protection and cancer therapeutics.
Keyphrases
- dna repair
- energy transfer
- dna damage
- circulating tumor
- single molecule
- induced apoptosis
- oxidative stress
- cell free
- high resolution
- papillary thyroid
- magnetic resonance imaging
- cell cycle arrest
- computed tomography
- squamous cell carcinoma
- magnetic resonance
- risk assessment
- cell death
- climate change
- human health
- radiation induced
- contrast enhanced