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Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division.

Phillip NußbaumDanguole Kureisaite-CizieneDom BelliniChris van der DoesMarko KojicNajwa TaibAnna YeatesMaxime TourteSimonetta GribaldoMartin LooseJan LöweSonja-Verena Albers
Published in: Nature microbiology (2024)
Cell division in all domains of life requires the orchestration of many proteins, but in Archaea most of the machinery remains poorly characterized. Here we investigate the FtsZ-based cell division mechanism in Haloferax volcanii and find proteins containing photosynthetic reaction centre (PRC) barrel domains that play an essential role in archaeal cell division. We rename these proteins cell division protein B 1 (CdpB1) and CdpB2. Depletions and deletions in their respective genes cause severe cell division defects, generating drastically enlarged cells. Fluorescence microscopy of tagged FtsZ1, FtsZ2 and SepF in CdpB1 and CdpB2 mutant strains revealed an unusually disordered divisome that is not organized into a distinct ring-like structure. Biochemical analysis shows that SepF forms a tripartite complex with CdpB1/2 and crystal structures suggest that these two proteins might form filaments, possibly aligning SepF and the FtsZ2 ring during cell division. Overall our results indicate that PRC-domain proteins play essential roles in FtsZ-based cell division in Archaea.
Keyphrases
  • single cell
  • cell therapy
  • gene expression
  • dna methylation
  • escherichia coli
  • single molecule
  • cell proliferation
  • bone marrow
  • small molecule
  • mass spectrometry
  • quantum dots
  • early onset
  • wild type