A Model of Blood Component-Heart Interaction in Cardiac Ischemia-Reperfusion Injury using a Langendorff-Based Ex Vivo Assay.

Johanna M MuessigSema KayaLuise MoellhoffJohanna NoelleLeonie Hidalgo ParejaMaryna MasyukNorbert GerdesJohn PernowMalte KelmChristian Jung

Published in: Journal of cardiovascular pharmacology and therapeutics (2019)

This Langendorff-based transfer model of cardiac I/R injury is a feasible, time-, and cost-effective model to evaluate the impact of blood components on myocardial infarction. The presented method facilitates loading of blood components of genetically modified mice to murine hearts of a different mouse strain, thus complementing time- and cost-intensive chimeric models and contributing to the development of novel targeted therapies.

Keyphrases

left ventricular
ischemia reperfusion injury
heart failure
oxidative stress
high throughput
cell therapy
stem cells
type diabetes
skeletal muscle
metabolic syndrome
insulin resistance
mesenchymal stem cells